您不能while True:在Tkinter事件循环所处的同一线程中启动循环。这样做将阻塞Tkinter的循环并导致程序冻结。
对于一个简单的解决方案，您可以Tk.after每秒钟左右在后台运行一个进程。下面是一个脚本来演示：
from Tkinter import *
running = True # Global flag
def scanning():
if running: # Only do this if the Stop button has not been clicked
print "hello"
# After 1 second, call scanning again (create a recursive loop)
root.after(1000, scanning)
def start():
"""Enable scanning by setting the global flag to True."""
global running
running = True
def stop():
"""Stop scanning by setting the global flag to False."""
global running
running = False
root = Tk()
root.title("Title")
root.geometry("500x500")
app = Frame(root)
app.grid()
start = Button(app, text="Start Scan", command=start)
stop = Button(app, text="Stop", command=stop)
start.grid()
stop.grid()
root.after(1000, scanning) # After 1 second, call scanning
root.mainloop()
当然，您可能希望将此代码重构为一个类并running成为其属性。另外，如果您的程序变得很复杂，那么研究Python的threading模块将是有益的，这样您的scanning函数可以在单独的线程中执行。
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