codecs模块正是您所需要的。这里详细
import codecs
def replace_line(file_name, line_num, text):
f = codecs.open(file_name, 'r', encoding='utf-8')
lines = f.readlines()
lines[line_num] = text
f.close()
w = codecs.open(file_name, 'w', encoding='utf-8')
w.writelines(lines)
w.close()
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