这为我工作：
def compare(File1,File2):
with open(File1,'r') as f:
d=set(f.readlines())
with open(File2,'r') as f:
e=set(f.readlines())
open('file3.txt','w').close() #Create the file
with open('file3.txt','a') as f:
for line in list(d-e):
f.write(line)
您需要比较设置的readlines，并找出file2中不存在的行。然后，您可以将这些行附加到新文件中。
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